Recombinant Mouse 2B4/CD244/SLAMF4 protein (His Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg1583
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse 2B4/CD244/SLAMF4 protein Gln20-Pro226 (Accession# Q07763-1) with a His tag at the C-terminus. |
| GeneID | 18106 |
| Accession | Q07763-1 |
| PredictedSize | 24.5 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs. CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function.
References:
1. Alexander David Barrow. et al. (2019) Front Immunol. 10:909. 2. M H Brown. et al. (1998) J Exp Med.188(11):2083-90. 3. Laura Agresta. et al. (2018) Front Immunol. 9:2809. 4. M H Brown. et al. (1998) J Exp Med. 188(11):2083-90.