Recombinant Mouse ACE protein (rFc Tag)

• Home
• Products
• Fusion Proteins
• AuthentiKine ELISA Kits
• Recombinant Mouse ACE protein (rFc Tag) (Eg3036)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3036

---

Validation Data Gallery

• 

  Purity of Recombinant Mouse ACE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse ACE protein Leu35-Gln1264 (Accession# P09470-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 11421
• Accession: P09470-1
• PredictedSize: 167.9 kDa
• SDS-PAGE: 140-200 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Angiotensin I Converting Enzyme (ACE) is a transmembrane zinc metallopeptidase that hydrolyzes peptide chains by removing the dipeptide from the C-terminus. ACE is a key component of the renin-angiotensin system (RAS), which plays an important role in blood pressure homeostasis through the production of the vasoconstrictor peptide angiotensin II. ACE also plays a role in fertility, immunity, hematopoiesis, and diseases such as obesity, fibrosis, and Alzheimer's disease dementia. ACE is relevant to immune regulation in that endogenous ACE has been detected in a variety of immune cell populations including macrophages and neutrophils, and its overexpression results in enhanced bactericidal and antitumor responses independent of angiotensin II.

References:

1. Oosthuizen D, et al. (2022). J Renin Angiotensin Aldosterone Syst. 2022:9028969.
2. Rahimi Z. (2012). J Nephropathol. 1(3):143-51.
3. Lubbe L,et al. (2020). Clin Sci (Lond). 134(21):2851-2871.