Speedy™ Mouse Adiponectin One-Step ELISA Kit
Cat no : SE60003
Synonyms
Adipoq, Acrp30, adipo, apM1
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse Adiponectin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue homogenate |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11450 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 96%-108% |
| Cell culture supernatant | 107% | 96%-123% |
| Tissue homogenate | 91% | 84%-101% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 477.3 | 14.4 | 3.0 |
| 2 | 8 | 125.0 | 4.5 | 3.6 |
| 3 | 8 | 63.0 | 2.1 | 3.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 495.4 | 24.9 | 5.0 |
| 2 | 16 | 127.8 | 6.3 | 4.9 |
| 3 | 16 | 64.1 | 3.1 | 4.8 |
Background Information
Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity. Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases. Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Adipoq, Acrp30, adipo, apM1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.