Recombinant Mouse ALPL protein (rFc Tag)

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• Recombinant Mouse ALPL protein (rFc Tag) (Eg2714)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2714

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Validation Data Gallery

• 

  Purity of Recombinant Mouse ALPL was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse ALPL protein Phe18-Gly501 (Accession# P09242) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 11647
• Accession: P09242
• PredictedSize: 79.5 kDa
• SDS-PAGE: 85-95 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa. ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI).This antibody can bind the four mentioned alkaline phosphatases.

References:

1. Sogabe N, et al. (2008). Biomed Res. 29(4):213-9. 
2. Fukushi-Irié M, et al. (2000). Biochem J. 15;348 Pt 3(Pt 3):633-42.