Recombinant Mouse ARMETL1 protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2845

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Validation Data Gallery

• 

  Purity of Recombinant Mouse ARMETL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse ARMETL1 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse ARMETL1 protein Leu27-Leu187 (Accession# Q8CC36) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 227526
• Accession: Q8CC36
• PredictedSize: 44.4 kDa
• SDS-PAGE: 40-48 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ARMETL1, also known as Cerebral Dopamine Neurotrophic Factor (CDNF), is a secreted protein with eight conserved cysteine residues. It is a novel neurotrophic factor with strong trophic activity on dopaminergic neurons, comparable to that of glial cell line-derived neurotrophic factor (GDNF). ARMETL1 is known for its neuroprotective and reparative effects, particularly in models of Parkinson's disease and stroke, and is currently in clinical trials for Parkinson's disease.

References:

1.Albert K, et al. (2019). Croat Med J. 60(2):99-108.
2.Lindholm P, et al. (2007). Nature. 448(7149):73-77. 
3.Lohoff FW, et al. (2009). Neurosci Lett. 453(3):199-203.