Recombinant Mouse CD200 protein (hFc Tag)(HPLC verified)

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• Recombinant Mouse CD200 protein (hFc Tag)(HPLC verified) (Eg0919)

Species

Mouse

Purity

>95 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

hFc Tag

Activity

EC50: 8-32 ng/mL

Cat no : Eg0919

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD200 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  Immobilized Mouse CD200R1 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD200 (hFc tag) with a linear range of 8-32 ng/mL.

• 

  The purity of Mouse CD200 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >95 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Immobilized Mouse CD200R1 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD200 (hFc tag) with a linear range of 8-32 ng/mL.

• Expression: HEK293-derived Mouse CD200 protein Gln31-Gly232 (Accession# O54901) with a human IgG1 Fc tag at the C-terminus.
• GeneID: 17470
• Accession: O54901
• PredictedSize: 48.5 kDa
• SDS-PAGE: 60-68 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells. CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage. The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses.

References:

1.G W McCaughan, et al. (1987) Immunogenetics. 25(5):329-35.
2.G J Wright, et al. (2000) Immunity. 13(2):233-42. 
3.Reginald Gorczynski, et al. (2004) J Immunol. 172(12):7744-9.
4.T P Rygiel, et al. (2012) Oncogene. 31(24):2979-88.