Recombinant Mouse B7-H3/CD276 protein (rFc Tag)

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• Recombinant Mouse B7-H3/CD276 protein (rFc Tag) (Eg3849)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3849

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Validation Data Gallery

• 

  Purity of Recombinant Mouse B7-H3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse B7-H3 protein Val29-Ala248 (Accession# Q8VE98) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 102657
• Accession: Q8VE98
• PredictedSize: 50.2 kDa
• SDS-PAGE: 60-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

B7 homolog 3 protein (B7-H3), also known as CD276, is an immune checkpoint molecule and a costimulatory/coinhibitory immunoregulatory protein that plays a dual role in the immune system. It was first cloned in 2001 from a cDNA library that was derived from human dendritic cells (DCs).

References:

1. Hofmeyer KA, et al. Proc Natl Acad Sci USA (2008) 105:10277–8. 
2. Chapoval AI, Chapoval AI, Nat Immunol (2001) 2:269–74.