Recombinant Mouse CD314/NKG2D protein (rFc Tag)

• Home
• Products
• Fusion Proteins
• AuthentiKine ELISA Kits
• Recombinant Mouse CD314/NKG2D protein (rFc Tag) (Eg3829)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3829

---

Validation Data Gallery

• 

  Purity of Recombinant Mouse CD314 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD314 protein Phe90-Val232 (Accession# O54709-1) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 27007
• Accession: O54709-1
• PredictedSize: 43.6 kDa
• SDS-PAGE: 45-55 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Natural killer group 2D (NKG2D) (CD314) is an activating receptor on NK cells, CD8+ T cells, NKT cells and γδ+ T cells. Interleukin (IL)-15-induced up-regulation of NKG2D on intraepithelial cytotoxic T cells (IELs) and NKG2D ligands on enterocytes is a hallmark in CD and is responsible for epithelial cell destruction and subsequent villous atrophy.

References:

1. Guy-Grand D, et al. Eur J Immunol. 1996;26:2248–2256.
2. Abadie V, et al. Semin Immunopathol. 2012;34:551–566.