Recombinant Mouse CD69 protein (rFc Tag) (HPLC verified)

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• Recombinant Mouse CD69 protein (rFc Tag) (HPLC verified) (Eg3835)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3835

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD69 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse CD69 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD69 protein Asn62-Arg199 (Accession# P37217) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 12515
• Accession: P37217
• PredictedSize: 43.0 kDa
• SDS-PAGE: 48-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD69 is a C-type lectin whose gene is encoded within the NK cell gene complex on chromosome 6 in mice and chromosome 12 in humans. CD69 promotes immune homeostasis by regulating the differentiation and function of Tregs. On the basis of CD69 expression, Tregs are classified into 2 subtypes: CD69+ and CD69– Tregs. CD69+ Tregs express higher levels of suppression markers such as CTLA-4, ICOS, CD38, and GITR than to CD69– or Cd69–/– Tregs, resulting in enhanced effector T cell suppression and tolerance.

References:

1. Cortes JR, et al. J Autoimmun. 2014;55:51–62.
2. Sanchez-Diaz R, et al. Mol Cell Biol. 2017;37(9):e00341.