Recombinant MouseCXCR4/CD184 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2231
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CXCR4 protein Met1-Arg40 (Accession# P70658) with a rabbit IgG Fc tag at the N-terminus. |
| GeneID | 12767 |
| Accession | P70658 |
| PredictedSize | 31.8 kDa |
| SDS-PAGE | 34-42 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. CXCR4 also functions as a coreceptor for HIV-1 entry.
References:
1. Teicher, Beverly A, and Simon P Fricker. Clinical cancer research : an official journal of the American Association for Cancer Research vol. 16,11 (2010): 2927-31. 2. Donzella, G A et al. Nature medicine vol. 4,1 (1998): 72-7. 3. Asfour, Inas et al. Hematology/oncology and stem cell therapy vol. 10,2 (2017): 63-69.