Recombinant Mouse CCL17/TARCprotein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3319
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse CCL17 protein Ala24-Pro93 (Accession# Q9WUZ6) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 20295 |
| Accession | Q9WUZ6 |
| PredictedSize | 33.9 kDa |
| SDS-PAGE | 35-43 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CCL17, also named as TARC, is a member of the CC chemokine family, and is highly expressed by thymus and other cells, including keratinocytes, endothelial cells, dendritic cells, bronchial epithelial cells and fibroblasts. CCL17 plays important roles in T cell development in thymus as well as in trafficking and activation of mature T cells. CCL17 induced chemotaxis and Ca2+ influx in the cells stably expressing CCR4, thereby demonstrating the ligand-receptor relationship between CCL17 and CCR4.
References:
1. ImaiT.etal. (1996) J BiolChem. 271:21514-1. 2. Sallusto F.etal. (1998) Eur J Immunol. 28:2760-9. 3. CampbellJJ.etal. (1999) Nature. 400:776-80. 4. Vestergaard C.etal. (1999) J Clin Invest. 104:1097-105. 5. Sekiya T.etal. (2000) J Immunol.165:2205-13. 6. YuB.etal. (2000) J DermatolSci.30:29-36.