Recombinant Mouse CD53 protein (rFc Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2684

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD53 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse CD53 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD53 protein Glu107-Asn181 (Accession# Q61451) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 12508
• Accession: Q61451
• PredictedSize: 34.2 kDa
• SDS-PAGE: 38-50 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD53, also known as OX44 or TSPAN25,a pan-leukocyte surface glycoprotein which spans the plasma membrane four times and is a member of the transmembrane 4 superfamily (TM4SF). Mouse CD53 is expressed on virtually all peripheral leukocytes, but not on cells outside the lymphoid or myeloid lineages.

References:

1.Dunlock VE. (2020) Med Microbiol Immunol. 209(4):545-552. 
2.Tomlinson MG, et al. (1995) Eur J Immunol. 25(8):2201-5.