Recombinant Mouse CD74 protein (mFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg4048

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD74 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD74 protein Gln56-Leu279 (Accession# P04441-1) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 16149
• Accession: P04441-1
• PredictedSize: 52.1 kDa
• SDS-PAGE: 52-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.

References:

1. Burton JD, et al. (2004). Clin Cancer Res.1;10(19):6606-11. 
2 .Su H, et al. (2017). Inflamm Res. 66(3):209-216. 
3 . Zhang L, et al. (2024). Cell Mol Life Sci.11;81(1):296. 
4.Li QL, et al. (2023). Front Cardiovasc Med. 12;9:1049143.