Recombinant Mouse Chromogranin A protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2760
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Chromogranin A protein Leu19-Gly463 (Accession# P26339) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 12652 |
| Accession | P26339 |
| PredictedSize | 75.5 kDa |
| SDS-PAGE | 70-78 kDa and 85-110 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells, which is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). Chromogranin A is one of the most used tumor markers in NET's (neuroendocrine tumors).
References:
1. Modlin, Irvin M et al. Annals of surgical oncology vol. 17,9 (2010): 2427-43. 2. Louthan, O. Folia biologica vol. 57,5 (2011): 173-81. 3. Nobels, F R et al. European journal of clinical investigation vol. 28,6 (1998): 431-40. 4. Campana, Davide et al. Journal of clinical oncology : official journal of the American Society of Clinical Oncology vol. 25,15 (2007): 1967-73.