Recombinant Mouse CRTAM/CD355 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2691

---

Validation Data Gallery

• 

  Purity of Recombinant Mouse CRTAM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) condition and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CRTAM protein Ala17-Ala284 (Accession# Q149L7-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 54698
• Accession: Q149L7-1
• PredictedSize: 55.5 kDa
• SDS-PAGE: 65-85 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion. CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes. It also regulates CD8+ T cell retention within the lymph node and eventually effector function.

References:

1.Yeh JH, et al. (2008) Cell. 7;132(5):846-59. 
2.Garay E, et al. (2010) J Cell Biochem. 1;111(1):111-22.
3.Takeuchi A, et al. (2015) J Exp Med. 11;213(1):123-38. 
4.Takeuchi A,et al. (2009) J Immunol. 1;183(7):4220-8.