Recombinant Mouse CXCL15 protein (rFc Tag) (HPLC verified)

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• Recombinant Mouse CXCL15 protein (rFc Tag) (HPLC verified) (Eg2776)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2776

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CXCL15 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse CXCL15 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CXCL15 protein Gln26-Ala167 (Accession# Q9WVL7) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 20309
• Accession: Q9WVL7
• PredictedSize: 42.4 kDa
• SDS-PAGE: 40-48 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CXCL15 is a murine chemokine that is not found in humans and for this reason has been poorly researched. CXCL15 is mainly expressed in murine lungs, and to a much lesser extent in the murine digestive tract and urogenital organs. The expression of this chemokine can be increased by chronic hypoxia, as shown by experiments on murine articular chondrocytes, where the overexpression of HIF-2α resulted in an elevated expression of CXCL15.

References:

1. Yue F. et al. (2014) Nature. 515:355–364.
2. Schmitz JM. et al. (2007) HistoChem. Cytochem. 55:515–524.
3. Huh YH. et al. (2015) Arthritis Res. Ther. 17:302.