Recombinant Mouse CXCL16 protein (rFc Tag) (HPLC verified)

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• Recombinant Mouse CXCL16 protein (rFc Tag) (HPLC verified) (Eg2846)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2846

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CXCL16 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse CXCL16 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CXCL16 protein Asn27-Pro114 (Accession# Q8BSU2) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 66102
• Accession: Q8BSU2
• PredictedSize: 36.0 kDa
• SDS-PAGE: 35-42 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in 30mM Tris,500mM arginine, pH 8.0. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CXC motif chemokine ligand 16 (CXCL16) is classified as an α sub-family chemokine because it has a CXC motif at the N-terminus. Nevertheless, this chemokine lacks the ELR motif, which is common in other pro-angiogenic CXC chemokines. CXCL16 is expressed in lymphoid organs such as the thymus, spleen, lymph nodes, and Peyer’s patches, but not in bone marrow. It is also expressed in the liver, lung, small intestine, and kidney.

References:

1. Korbecki J, et al. (2021) Int J Mol Sci. 28;22(7):3490.
2. Matloubian M, et al. (2000) Nat. Immunol. 298–304.
3. Wilbanks A, et al. (2001) J. Immunol. 66:5145–5154.