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Recombinant Mouse EPCR/CD201 protein (rFc Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg1517

Validation Data Gallery

  • Purity of Recombinant Mouse EPCR was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse EPCR was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse EPCR was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse EPCR was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse EPCR protein Leu18-Ser214 (Accession# Q64695) with a rabbit IgG Fc tag at the C-terminus.
GeneID 19124
Accession Q64695
PredictedSize 48.7 kDa
SDS-PAGE 55-70 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

EPCR (endothelial cell protein C receptor) is a type 1 transmembrane protein that is primarily found on endothelial cells. It exhibits sequence and three-dimensional structural homology with the major histocompatibility class 1/CD1 family of proteins, particularly CD1d. It binds activated protein C and enhances protein C activation by the thrombin-thrombomodulin complex. It plays a role in the protein C pathway controlling blood coagulation.

References:

1. Jian-Ming Gu, et al. (2002). J Biol Chem. Nov 8;277(45):43335-43.
2. Usha R Pendurthi, L Vijaya Mohan Rao. (2018). Curr Opin Hematol. May;25(3):219-226.
3. Jian-Ming Gu, et al. (2002). J Biol Chem. Nov 8;277(45):43335-43.