Recombinant Mouse FSTL1 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2859

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Fstl1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse FSTL1 protein Glu19-Ile306 (Accession# P15144) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 14314
• Accession: Q62356
• PredictedSize: 58.5 kDa
• SDS-PAGE: 65-75 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Follistatin-like 1 (FSTL1), initially discovered as a TGF-β1-induced gene, encodes a 308 amino acid secreted 45-55 kDa glycoprotein with a follistatin domain and two non-functional calcium-binding motifs. FSTL1 has been reported to be involved in the fate determination and maturation of epithelial cells. Ablation of the FSTL1 gene in the mouse results in several structural-developmental defects and neonatal lethality due to respiratory failure, demonstrating its functional importance. FSTL1 has been reported to exhibit both pro- and anti-inflammatory actions, with a specific anti-apoptotic and protective effect in cardiac or renal cell injury. detected several isoforms of FSTL1 with molecular masses of 40 to 48 kD which differs from the 50- to 55-kD products detected.

References:

1. Mouillet JF. et al. (2015). Placenta. 36(11):1231-8. 
2. Sylva M. et al. (2013). Birth Defects Res C Embryo Today. 99(1):61-9. 
3. Umezu T. et al. (2010). Proc Natl Acad Sci U S A. 107(10):4601-6.