Recombinant Mouse HO-1/HMOX1 protein (His Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
His Tag
Activity
not tested
Cat no : Eg0938
Validation Data Gallery
Product Information
Purity | >90 %, SDS-PAGE |
Endotoxin | <0.1 EU/μg protein, LAL method |
Activity |
Not tested |
Expression | HEK293-derived Mouse HO-1 protein Met1-Thr266 (Accession# P14901) with a His tag at the C-terminus. |
GeneID | 15368 |
Accession | P14901 |
PredictedSize | 31.5 kDa |
SDS-PAGE | 27-30 kDa, reducing (R) conditions |
Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed. Heme oxygenase-1 (HO-1) is expressed in many tissues vascular smooth muscle cells, and endothelial cells and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, inflammatory cytokines, oxidative stress, heat shock, heavy metals and endotoxin.
References:
1. Ma, T.et al. (2016). Exp Eye Res146,318-329. 2.Loboda, A.et al. (2016). Cell MolLife Sci73,3221-47. 3. Blann, AD.et al. (2011). Prostate Cancer Prostatic Dis14,114-7.