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Recombinant Mouse HO-1/HMOX1 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0938

Validation Data Gallery

  • Purity of Recombinant Mouse HO-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse HO-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse HO-1 protein Met1-Thr266 (Accession# P14901) with a His tag at the C-terminus.
GeneID 15368
Accession P14901
PredictedSize 31.5 kDa
SDS-PAGE 27-30 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed. Heme oxygenase-1 (HO-1) is expressed in many tissues vascular smooth muscle cells, and endothelial cells and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, inflammatory cytokines, oxidative stress, heat shock, heavy metals and endotoxin.

References:

1. Ma, T.et al. (2016). Exp Eye Res146,318-329.
2.Loboda, A.et al. (2016). Cell MolLife Sci73,3221-47.
3. Blann, AD.et al. (2011). Prostate Cancer Prostatic Dis14,114-7.