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Recombinant Mouse Hpx/Hemopexin protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2829

Validation Data Gallery

  • Purity of Recombinant Mouse Hpx was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Hpx was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse Hpx was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse Hpx was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Hpx protein Ser24-Gln460 (Accession# Q91X72) with a rabbit IgG Fc tag at the C-terminus.
GeneID 15458
Accession Q91X72
PredictedSize 74.9 kDa
SDS-PAGE 80-100 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Hemopexin (HPX) is a 60-kDa plasma glycoprotein mainly expressed by the liver, although the nervous system, skeletal muscle, retina, and kidney are also recognized Hx synthesis sites. HPX is the plasma protein with the highest binding affinity to heme among known proteins. It is mainly expressed in liver, and belongs to acute phase reactants, the synthesis of which is induced after inflammation.

References:

1.Fiorito V, Tolosano E. (2022). Int J Mol Sci. 23(2):997.
2.Tolosano E, Altruda F. (2002). DNA Cell Biol. 21(4):297-306.