Atlantic Blue™ Mouse IgG1 Isotype Control (1F8D3)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

1F8D3

Cat no : AB-66360-1

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

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Validation Data Gallery

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  FC experiment of human PBMCs using AB-66360-1

  1X10^6 human PBMCs were surface stained with 0.2 ug Atlantic Blue™ Anti-Mouse IgG1 Isotype Control (AB-66360-1, Clone:1F8D3) (red), Atlantic Blue™ Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-66360-1 targets IgG1 Isotype Control in FC applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: IgG1 Isotype Control
• Conjugate: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 404 nm / 458 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).