CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8D3

Cat no : CL488-66360-1

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

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Validation Data Gallery

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  FC experiment of human PBMCs using CL488-66360-1

  1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-IgG1 Isotype Control (CL488-66360-1, Clone:1F8D3) (red), CL488 Anti-Human CD35 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of A431 using CL488-66360-1

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Mouse IgG1 isotype control (CL488-66360-1, Clone: 1F8D3) (red), or 0.4 ug CoraLite® Plus 488 Anti-Human FKBP8 (CL488-66690, Clone: 1C1G12) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: human PBMCs, A431 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66360-1 targets IgG1 Isotype Control in FC applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: IgG1 Isotype Control
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).