Mouse IgG3 isotype control Monoclonal antibody

• 

  WB analysis using 66360-4-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-4-Ig (KLH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Mouse IgG3 isotype control Monoclonal Antibody for WB, ELISA

Cat No. 66360-4-Ig

Clone No.1H4A5

Publications(2)

Host / Isotype

Mouse / IgG3

Reactivity

megathura crenulata and More (1)

Applications

WB, CoIP, RIP, ELISA

Synonyms

Mouse IgG3 control, IgG3 isotype, mouse IgG control

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Tested Applications

Positive WB detected in

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

66360-4-Ig targets Mouse IgG3 isotype control in WB, CoIP, RIP, ELISA applications and shows reactivity with megathura crenulata samples.

• Tested Reactivity: megathura crenulata
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Mouse IgG3 isotype control
• RRID: AB_2923630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG3. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	RIP	Biochim Biophys Acta Mol Basis Dis; IGF2BP1 exacerbates neuroinflammation and cerebral ischemia/reperfusion injury by regulating neuronal ferroptosis and microglial polarization; Authors - Youjun Li
mouse	CoIP	Neurosci Bull; USP47 Regulates Excitatory Synaptic Plasticity and Modulates Seizures in Murine Models by Blocking Ubiquitinated AMPAR Degradation; Authors - Juan Yang