Recombinant Mouse IL18BP protein (rFc Tag)

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• Recombinant Mouse IL18BP protein (rFc Tag) (Eg3158)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3158

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Validation Data Gallery

• 

  Purity of Recombinant Mouse IL18BP was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse IL18BP protein Thr29-Ala193 (Accession# Q9Z0M9) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 16068
• Accession: Q9Z0M9
• PredictedSize: 44.0 kDa
• SDS-PAGE: 48-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-18 Binding Protein (IL18BP) is a soluble protein that acts as a natural antagonist of interleukin-18 (IL-18), a pro-inflammatory cytokine involved in immune responses. It binds to IL-18 with high affinity, preventing IL-18 from interacting with its receptors (IL-18Rα and IL-18Rβ) and thus inhibiting its biological activity. This regulation is crucial for maintaining immune homeostasis and preventing excessive inflammation. IL18BP is a biomarker for disease activity and severity, as well as a therapeutic target in cancer immunotherapy.

References:

1.Novick D. et al. (1999). Immunity. 10(1):127-136.
2.Kim SH. et al. (2000). Proc Natl Acad Sci U S A. 97(3):1190-1195.
3.Belkaya S. et al. (2019). J Exp Med. 216(8):1777-1790.
4.Zhou T. et al. (2020). Nature. 583(7817):609-614.