Recombinant Mouse IL-19 protein (rFc Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2844

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Validation Data Gallery

• 

  Purity of Recombinant Mouse IL-19 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

• 

  The purity of Mouse IL-19 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse IL-19 protein Leu25-Ala176 (Accession# Q8CJ70) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 329244
• Accession: Q8CJ70
• PredictedSize: 43.6 kDa
• SDS-PAGE: 50-60 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-19 is found within the IL-10 family gene cluster: IL-10, -19, -20, -22, -24 (MDA-7), -26 (AK155), -28, and -29. IL-19 derived primarily from macrophages and, to a lesser extent, from B cells and nonimmune cells including epithelial cells, endothelial cells, skin keratinocytes, and fetal membranes. Many reports showed that IL-19 has proinflammatory effects.

References:

1. Wolk K, et al. (2002). Journal of Immunology. 168(11):5397-5402. 
2. Menon R, et al. (2006). Journal of Maternal-Fetal and Neonatal Medicine. 19(4):209-214. 
3. Chen YY, et al. (2013). Clin Dev Immunol. 2013:294320.