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Recombinant Mouse IL-28A protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3169

Validation Data Gallery

  • Purity of Recombinant Mouse IL-28A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-28A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse IL-28A was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse IL-28A was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse IL-28A protein Asp20-Val193 (Accession# Q4VK74) with a rabbit IgG Fc tag at the C-terminus.
GeneID 330496
Accession Q4VK74
PredictedSize 45.7 kDa
SDS-PAGE 48-55 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-28A belongs to the type III IFNs (also designated IFN-λs) and functions as an interferon but is structurally similar to the IL-10 family. IL-28A is not constitutively expressed but can be induced in different hematopoietic and non-hematopoietic cells. IL-28A binds their cellular receptor complex and mainly activates signal transduction via the JAK/STAT pathway. IL-28A exerts an anti-inflammatory and pro-inflammatory role.

References:

1.Witte K. et al. (2010). Cytokine Growth Factor Rev. 21(4):237-251. 
2.Ouyang W. et al. (2011). Annu Rev Immunol. 29:71-109.
3.Ni S. et al. (2022). Int Immunopharmacol. 108:108881.