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Recombinant Mouse CD25/IL2RA protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3087

Validation Data Gallery

  • Purity of Recombinant Mouse CD25 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD25 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CD25 protein Glu22-Lys236 (Accession# P01590) with a rabbit IgG Fc tag at the C-terminus.
GeneID 16184
Accession P01590
PredictedSize 50.7 kDa
SDS-PAGE 55-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD25, the α chain of interleukin-2 receptor (IL2RA), is predominantly expressed on the surface of immune-related cells, including activated T cells, B cells, and NK cells. For cancers, CD25 is expressed at high levels in many types of hematological malignancies, but at low levels in most solid tumors. CD25 is also highly expressed in activated circulating immune cells and regulatory T cells (Tregs). CD25 is one of the non-MHC II genes associated with some autoimmune diseases such as systemic sclerosis and systemic lupus erythematosus. CD25 as a potential target for the depletion of Tregs is critical in developing new immunotherapeutic strategies.

References:

1.Hinks A. et al. (2009) Arthritis Rheum. 60(1):251-257.
2.Alsahebfosoul F. et al. (2017) Mult Scler Relat Disord. 18:117-118.
3.Peng Y. et al. (2023) Int J Cancer. 152(7):1290-1303. 
4.Luo H. et al. (2023) Front Immunol. 14:1247710. 
5.Spolski R. et al. (2023) Sci Immunol. 8(89):eadi8217.