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Recombinant Mouse JAM-3 protein (rFc Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2661

Validation Data Gallery

  • Purity of Recombinant Mouse JAM-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse JAM-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse JAM-3 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse JAM-3 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse JAM-3 protein Glu30-Asn241 (Accession# Q9D8B7) with a rabbit IgG Fc tag at the C-terminus.
GeneID 83964
Accession Q9D8B7
PredictedSize 49.9 kDa
SDS-PAGE 50-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Junctional adhesion molecules (JAMs) are integral membrane proteins belonging to the immunoglobulin (Ig) superfamily. JAMs are expressed by leukocytes, platelets, endothelial, and epithelial cells and localized at the tight junction of polarized cells and on the cell surface of leukocytes. JAM-3, also known as JAM-C, is expressed on human platelets. JAM-3 is a novel counterreceptor for the integrin Mac-1 (CD11b/CD18) thereby playing a critical role for platelet–leukocyte interactions.

References:

1.Ebnet K, et al. (2004) J Cell Sci.1;117(Pt 1):19-29. 
2.Garrido-Urbani S, et al. (2014) Cell Tissue Res. 355(3):701-15.
3.Santoso S, et al. (2003) J Exp Med.196(5):679-91.