Recombinant Mouse CD107b/LAMP2 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2029

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD107b was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD107b protein Leu26-Asn379 (Accession# P17047-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 16784
• Accession: P17047-1
• PredictedSize: 65.5 kDa
• SDS-PAGE: 85-120 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis. Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells. LAMP2 is involved in lysosomal stability and autophagy. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis.

References:

1. R Kundra, S Kornfeld. (1999) J Biol Chem. Oct 22;274(43):31039-46.
2. Eeva-Liisa Eskelinen, et al. (2002) Mol Biol Cell. Sep;13(9):3355-68.
3. V Sarafian, et al. (1998) Int J Cancer. Jan 5;75(1):105-11.