Recombinant Mouse Lepr protein (rFc Tag) (HPLC verified)

• Home
• Products
• Fusion Proteins
• AuthentiKine ELISA Kits
• Recombinant Mouse Lepr protein (rFc Tag) (HPLC verified) (Eg2930)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2930

---

Validation Data Gallery

• 

  Purity of Recombinant Mouse Lepr was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse Lepr was greater than 90% as determined by SEC-HPLC.

---

Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Lepr protein Leu22-Gly839 (Accession# P48356-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 16847
• Accession: P48356-1
• PredictedSize: 118.2 kDa
• SDS-PAGE: 120-180 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction. Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3. LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity.

References:

1.Cottrell EC. et al. (2012). Handb Exp Pharmacol. (209):3-21.
2.Tartaglia LA. (1997). J Biol Chem. 272(10):6093-6096. 
3.Baskin DG. et al. (1999). J Histochem Cytochem. 47(3):353-362.