Recombinant mouse PTPRJ/CD148 protein

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• Recombinant mouse PTPRJ/CD148 protein (Eg2648)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2648

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Validation Data Gallery

• 

  Purity of Recombinant Mouse PTPRJ/CD148 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse PTPRJ/CD148 protein Arg29-Ile874 (Accession# Q64455) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 19271
• Accession: Q64455
• PredictedSize: 117.7 kDa
• SDS-PAGE: 135-250 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

PTPRJ (Protein Tyrosine Phosphatase Receptor Type J), also known as CD148 or density-enhanced phosphatase-1 (DEP-1), belongs to the type III receptor-type PTP (RPTP) subfamily and has been identified as a negative regulator of insulin signaling in peripheral tissues and is overexpressed in obese mice. Ptprj is the most abundant RPTP in platelets and megakaryocytes. Its structure consists of a single intracellular catalytic PTP domain, the transmembrane domain, and an extracellular domain composed of 9 fibronectin type III–like repeats. Ptprj plays critical roles in retinocollicular projections by controlling the repulsive signal by Eph and c-Abl activities.

References:

1. Shintani T, et al. (2017). Sci Rep. Sep 14;7(1):11627.
2. Yu Y, et al. (2018). J Neurosci. Sep 26;38(39):8345-8363.et al.
3. Marconi C,et al. (2019). Blood. Mar 21;133(12):1346-1357. 
4. Ulke J, et al. (2024). J Neuroendocrinol. 36(12):e13446.