Recombinant Mouse RAB5A protein (rFc Tag)

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• Recombinant Mouse RAB5A protein (rFc Tag) (Eg9093)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg9093

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Validation Data Gallery

• 

  Purity of Recombinant Mouse RAB5A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse RAB5A protein Met1-Asn215 (Accession# Q9CQD1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 271457
• Accession: Q9CQD1
• PredictedSize: 49.2 kDa
• SDS-PAGE: 48-54 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

RAB5A is a core member of the Rab family within the Ras superfamily of small GTPases and a marker protein for early endosome formation. Localized in the cell membrane and early endosome membrane, RAB5A acts as a molecular switch regulating the docking and fusion of endocytic vesicles, making it a key regulator of the endocytic pathway.

References:

1. Itoh, Takashi et al. Genes to cells : devoted to molecular & cellular mechanisms vol. 11,9 (2006): 1023-37.
2. Barbosa, M D et al. Mammalian genome : official journal of the International Mammalian Genome Society vol. 7,2 (1996): 166-7.
3. Cong, Xiao Xia et al. Cell death and differentiation vol. 27,8 (2020): 2344-2362.