Recombinant Mouse Reg2 protein (rFc Tag) (HPLC verified)

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• Recombinant Mouse Reg2 protein (rFc Tag) (HPLC verified) (Eg2693)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2693

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Reg2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Mouse Reg2 was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Reg2 protein Gln23-Ala173 (Accession# Q08731) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 19693
• Accession: Q08731
• PredictedSize: 42.9 kDa
• SDS-PAGE: 40-46 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Regenerating islet-derived 2 (Reg2) is a trophic factor that stimulates β-cell replication and protects against islet destruction. Reg2 also serves as an islet autoantigen that induces autoimmune assaults on the islets and worsens diabetic progression. Injection of Reg2 protein into NOD mice can delay the course of diabetes at an early stage and improve the anti-apoptosis ability of pancreatic β cells.

References:

1.Zhou YH,, et al. (2024) Biochem Pharmacol. 227:116444.
2.Yu L, et al. (2019) Diabetes Obes Metab. 21(5):1209-1222.
3.Huszarik K, et al. (2010) J Immunol. 185(9):5120-9.