Recombinant Mouse SEMA4A protein (rFc Tag)

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• Recombinant Mouse SEMA4A protein (rFc Tag) (Eg2680)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2680

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Validation Data Gallery

• 

  Purity of Recombinant Human SEMA4A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse SEMA4A protein Thr33-His682 (Accession# Q62178) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 20351
• Accession: Q62178
• PredictedSize: 97.7 kDa
• SDS-PAGE: 80-110 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Sema4A, the membrane-bound class 4 semaphorin, belongs to semaphorins superfamily. Sema4A funtions as potent axonal repellants in the nervous system, and is involved in vascular development. Two species of Sema4A with molecular weights of about 150 and 120 kDa were observed. The antibody recognizes the Sema4A protein, which is of appropriate molecular size (~120 kDa) for the glycosylated protein.

References:

1. Leitner DF. et al. (2015). Int J Parasitol. 45(12):797-808. 
2. Toyofuku T. et al. (2007). EMBO J. 7;26(5):1373-84. 
3. Leitner DF. et al. (2015). ASN Neuro. 29;7(3):1759091415587502.