Recombinant Mouse Semaphorin 4D/CD100 protein (rFc Tag)

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• Recombinant Mouse Semaphorin 4D/CD100 protein (rFc Tag) (Eg1432)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1432

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Semaphorin 4D was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Semaphorin 4D protein Phe24-Arg733 (Accession# O09126) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 20354
• Accession: O09126
• PredictedSize: 105.1 kDa
• SDS-PAGE: 110-150 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins. CD100 is a protein from the semaphorin family with important roles in the vascular, nervous and immune systems. It may be found as a membrane bound dimer or as a soluble molecule originated by proteolytic cleavage. CD100 is expressed by most cells of the hematopoietic system, including lymphoid cells such as B and T lymphocytes and natural killer cells, as well as myeloid cells (neutrophils, platelets, and monocytes) and endothelial cells, and its expression usually increases upon activation).

References:

1.Luque MCA. et al. (2018) Front Cardiovasc Med. 5:136.
2.Luque MC. et al. (2015) Mol Immunol. 67(2 Pt B):559-67.
3.Elhabazi A. et al. (2001) J Immunol. 166(7):4341-7.