Recombinant Mouse Serpina1a protein (rFc Tag)

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• Recombinant Mouse Serpina1a protein (rFc Tag) (Eg3047)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3047

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Serpina1a was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Serpina1a protein Glu25-Lys413 (Accession# P07758) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 20700
• Accession: P07758
• PredictedSize: 69.5 kDa
• SDS-PAGE: 68-85 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Serpina1a alo known as Alpha-1-antitrypsin (A1AT), it is an acute phase serine proteinase inhibitor, which is synthesized and secreted as a glycoprotein by hepatocytes and alveolar macrophages in humans to protect tissue from degradation by endogenous neutrophil elastase (NE). Severe A1AT deficiency is a congenital disorder, which occurs due to certain missense point mutations in the serum protease inhibitor, member 1 (SERPINA1) gene. The most predominant of these mutations is the Z-mutation, accounting for >95% of clinical A1AT deficiency cases. This point mutation results in substitution of glutamic acid at position 342 with lysine (E342K), which destabilizes the adjacent reactive centre loop (RCL).

References:

1. Eggenschwiler R. et al. (2019) Sci Rep. 9(1):7486. 
2. Murphy MP. et al. (2024) Am J Physiol Lung Cell Mol Physiol. 327(4):L600-L606. 
3. Zieger M. et al. (2022) Mol Ther Methods Clin Dev. 25:425-438.