Recombinant Mouse Antithrombin III protein (mFc Tag)

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• Recombinant Mouse Antithrombin III protein (mFc Tag) (Eg3967)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg3967

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Antithrombin III was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Antithrombin III protein His33-Asn465 (Accession# P32261) with a mouse IgG Fc tag at the N-terminus.
• GeneID: 11905
• Accession: P32261
• PredictedSize: 76.6 kDa
• SDS-PAGE: 75-85 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Serpin C1 is a plasma protease inhibitor belonging to the serpin superfamily. It plays a critical role in regulating the blood coagulation cascade and the complement system by inhibiting various activated serine proteases of the coagulation system, including thrombin. It is synthesized in the liver and secreted into the bloodstream, circulating as an inhibitor of serine proteases. Serpin C1 is the major control serpin of the kallikrein-kinin system (KKS), and its deficiency can lead to uncontrolled activation of KKS, resulting in the generation of the vasoactive peptide bradykinin, which is associated with hereditary angioedema (HAE).

References:

1.Drouet C. et al. (2022). Front Allergy. 3:835503.
2.Ponard D. et al. (2020). Hum Mutat. 41(1):38-57.