Recombinant Mouse Siglec1/CD169 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg5002
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Siglec1 protein Thr20-Asn510 (Accession# Q62230-1) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 20612 |
| Accession | Q62230-1 |
| PredictedSize | 79.8 kDa |
| SDS-PAGE | 85-100 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
CD169, also known as sheep erythrocyte receptor (SER), Sialoadhesin (Sn) or Siglec-1, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily. CD169 is a single-pass transmembrane protein containing a long extracellular domain, a transmembrane region, and a short cytoplasmic tail. It is primarily expressed on the surface of specific macrophage subsets and its precursor monocytes, as well as on some DCs. CD169 binds to sialic acid-containing glycoconjugates, particularly those with α(2,3)-linkages. It is involved in immune regulation, pathogen recognition, and cell-cell interactions.
References:
1. Crocker PR, Gordon S. (1986) J Exp Med. 164(6):1862-1875. 2. Hartnell A, et al. (2001) Blood. 97(1):288-296. 3. Liu Y, Xia Y, Qiu CH. (2021) Biomed Rep. 14(2):26.