Recombinant Mouse TNFSF12 protein (mFc Tag)

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• Recombinant Mouse TNFSF12 protein (mFc Tag) (Eg3599)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg3599

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Validation Data Gallery

• 

  Purity of Recombinant Mouse TNFSF12 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse TNFSF12 protein Arg105-His249 (Accession# O54907) with a mouse IgG Fc tag at the N-terminus.
• GeneID: 21944
• Accession: O54907
• PredictedSize: 43.7 kDa
• SDS-PAGE: 44-50 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

TNFSF12 (Tumor necrosis factor ligand superfamily member 12), also known as TWEAK (TNF-related weak inducer of apoptosis), belongs to the TNF superfamily and is expressed in a variety of tissues and by certain types of immune cells and cancer cell lines, which could activate the processing of initiator caspase-8 as well as caspase-9 and lead to the extrinsic and intrinsic apoptosis. co-culturing with alveolar macrophages or treating with TNFSF12 substantially enhanced fbroblast cell proliferation and resulted in increased expression of extracellular matrix (ECM) and fbrosis-related factors.

References:

1.  Chen J, et al. (2024). Am J Transl Res. Aug 15;16(8):4120-4133.
2.  Guo L, et al. (2024). J Transl Med. Jul 29;22(1):698.