ChromoTek Myc-Trap® 2.0 Magnetic Agarose, Kit for Immunoprecipitation

Validation Data Gallery

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  Immunoprecipitation of GFP-Myc fusion protein from HEK293T cells using Myc-Trap® 2.0 Magnetic Agarose. IP was done using both untransfected (mock) and transfected (GFP-myc) cells. I: Input, F: Flow-through, B: Bound.

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  Comparison of pulldown efficacy between the Myc-Trap® 2.0 Magnetic Agarose (left) and the original Myc-Trap Magnetic Agarose (right). Both products were used to immunoprecipitate GFP-myc fusion proteins from untransfected (mock) and transfected (GFP-myc) HEK293T cells. The Myc-Trap 2.0 has a higher binding capacity and is able to pull down more GFP-Myc protein than the Myc-Trap. Pulldowns with the Myc-Trap 2.0 Magnetic Agarose also show significantly reduced background.

The ChromoTek Myc-Trap® 2.0 Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-Myc NANOBODY®/VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of Myc-fusion proteins from cell extracts of various organisms.

Cat No. yt2mak

Publications(1)

Specificity

Myc-tag

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Synonyms

Myc tag, Myc, Myc-Trap, EQKLISEEDL

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Product Information

The ChromoTek Myc-Trap® 2.0 Magnetic Agarose, Kit for Immunoprecipitation consists of an anti-Myc NANOBODY®/VHH, which is coupled to magnetic agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of Myc-fusion proteins from cell extracts of various organisms.

• Description: The Myc-Trap 2.0 Magnetic Agarose, Kit for Immunoprecipitation contains Myc-Trap 2.0 Magnetic Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of myc-tagged proteins.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to the Myc-tag (sequence EQKLISEEDL) at the N-terminus, ; C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
• Binding Capacity: 20 μg of recombinant myc-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5, 0.1 mg/ml ChromoTek 2x Myc-peptide (2yp) in PBS pH 7.4
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.1% SDS, 1 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): 770 nM
• Compatibility with mass spectrometry: The Myc-Trap 2.0 is optimized for on-bead digestion. For the application note, please click here:; On-bead digest protocol for mass spectrometry
• RRID: AB_3717367
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

• Component: Description
• Myc-Trap 2.0 Magnetic Agarose: 20 reactions (500 µl)
• Lysis buffer: Optimized for cytoplasmic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution

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Publications

Application	Title
CoIP	Oncogene; Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress; Authors - Rosanna J Wilkins