N-cadherin Recombinant monoclonal antibody

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• N-cadherin Recombinant monoclonal antibody (82968-1-RR)

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• KD/KO Validated

N-cadherin Uni-rAb^® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, ELISA

Cat No. 82968-1-RR Copied!

Clone No. 230259H5

Publications (9)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IHC-Autostainer, IF/ICC, ELISA

Synonyms

230259H5, Cadherin 2, Cadherin-2, CD325, CDH2

• 

  WB analysis using 82968-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82968-1-RR (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 82968-1-RR

  WB result of N-cadherin antibody (82968-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded Human Heart Muscle slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 40x lens).Heat mediated antigen retrieval with Tris EDTA (pH 9.0) buffer. Staining was performed on the Biocare NeoPATH Pro. The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of Cholangiocarcinoma using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded Cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human heart using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human appendicitis using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human brain using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of Cholangiocarcinoma using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded Cholangiocarcinoma tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 82968-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 82968-1-RR (N-cadherin antibody) at dilution of 1:4000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 82968-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 82968-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 82968-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 82968-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using N-cadherin antibody (82968-1-RR, Clone: 230259H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 82968-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82968-1-RR against Human N-cadherin were performed. The affinity constant is 0.464 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC-Autostainer detected in: human heart tissue, Cholangiocarcinoma tissue, human liver tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human appendicitis tissue, human liver cancer tissue, human ovary cancer tissue, human brain tissue, Cholangiocarcinoma tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:50-1:500
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82968-1-RR targets N-cadherin in WB, IHC, IHC-Autostainer, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0701

  Product name: Recombinant Human N-Cadherin protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 160-724 aa of NM_001792.4

  Sequence: DWVIPPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNPTYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNAVYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPPQSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLSDPANWLKIDPVNGQITTIAVLDRESPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLDINDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRLNGDFAQLNLKIKFLEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIVGAGLGTGA

• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 100 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001792.4
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• RRID: AB_3670715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P19022-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

Publications

What published studies show

The N-cadherin Recombinant monoclonal antibody (230259H5), catalog number 82968-1-RR, has 9 citations. These appear in journals including Nature Microbiology, Frontiers in Immunology, European Journal of Medical Research, Journal of Cerebral Blood Flow & Metabolism, Biomedicines, Current Issues in Molecular Biology, and Biochemical and Biophysical Research Communications. Research fields span gallbladder cancer, endometrial cancer, brain arteriovenous malformations, oral cancer ferroptosis, prostate cancer, pancreatic cancer, immune microenvironment remodeling, and Wnt/β-catenin signaling.

Automatically generated from the 9 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Nat Microbiol; Bacteroides fragilis toxin promotes gall bladder cancer in mice.; Authors - Jun Chen
		Front Immunol; Prognostic significance and immune infiltration analysis of HMGA2 in endometrial cancer; Authors - Peng Jiang
human	WB	Front Immunol; The prognostic significance of JAML and its role in remodeling the immune microenvironment via the cGAS-STING pathway in endometrial cancer.; Authors - Chenfan Tian
human	WB	Eur J Med Res; The S1PR1-CCN1 axis drives endothelial-to-mesenchymal transition and vascular instability in brain arteriovenous malformations.; Authors - Ganglei Li
		J Cereb Blood Flow Metab; EXPRESS: NSUN6-mediated 5-methylcytosine modification of USP2-AS1 drives Wnt/β-catenin signaling in brain arteriovenous malformations.; Authors - Ganglei Li
human	WB	Biomedicines; Inhibition of TPI1 Sensitizes Cisplatin-Resistant Oral Cancer to Ferroptosis; Authors - Dandan Wang