Tested Applications
| Positive IF/ICC detected in | HeLa cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-82968 targets N-cadherin in IF/ICC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
| Calculated Molecular Weight | 100 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | NM_001792.4 |
| Gene Symbol | N-cadherin |
| Gene ID (NCBI) | 1000 |
| RRID | AB_3673157 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19022-1 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also includes E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 N-cadherin antibody CL488-82968 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The N-cadherin Recombinant monoclonal antibody (catalog no. CL488-82968) has 1 citation. It appears in Chem Biomed Imaging (Impact Factor 6.7), published in 2026 by Gil A. Gonzalez. The study, titled "Reconfiguration of the Microtubule Network and Dynamics in Hypoxic Cancer Cells," falls within the research fields of cancer biology and cellular dynamics under hypoxic conditions.
| Species | Application | Title |
|---|---|---|
Chem Biomed Imaging Reconfiguration of the Microtubule Network and Dynamics in Hypoxic Cancer Cells. |
