Na N4-acetylcytidine Monoclonal antibody, PBS Only

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  ELISA experiment of N4-acetylcytidine using 68498-1-Ig (same clone as 68498-1-PBS)

  Indirect ELISA and competitive ELISA results show that this antibody is specific to ac4C (N4-acetylcytidine). Indirect ELISA (blue curve, refer to top X-right Y axis) was performed by coating BSA conjugated ac4C at 50ng/well followed by blocking with 5% non fat milk. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Ac4C or its structure analogue compounds was mixed in 20ng/mL primary antibody (red curve, refer to bottom X-left Y axis). This data was developed using the same antibody clone with 68498-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of HeLa using 68498-1-Ig (same clone as 68498-1-PBS)

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 5% milk and blotted with N4-acetylcytidine antibody 68498-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG1 isotype control Monoclonal antibody 66360-1-Ig at the same dose. This data was developed using the same antibody clone with 68498-1-PBS in a different storage buffer formulation.

N4-acetylcytidine Monoclonal Antibody for Dot Blot, ELISA, Indirect ELISA

Cat No. 68498-1-PBS

Clone No.3C2C2

Host / Isotype

Mouse / IgG1

Reactivity

chemical compound

Applications

Dot Blot, ELISA, Indirect ELISA

Synonyms

C11H15N3O6, Ac-rC, Acetylcytidine, ACETYL CYTOSINE, Ac4C

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Product Information

68498-1-PBS targets N4-acetylcytidine in Dot Blot, ELISA, Indirect ELISA applications and shows reactivity with chemical compound samples.

• Tested Reactivity: chemical compound
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Compound

• Full Name: N4-acetylcytidine
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

N4-Acetylcytidine, CasNo. 3768-18-1, is a modified nucleoside and endogenous urinary nucleoside product of the degradation of tRNA, 18s rRNA and mRNA. N4-Acetylcytidine is a biological marker for various cancers with elevated concentrations present in urine. N4-Acetylcytidine is also a partially protected cytidine and therefore can be used as a synthetic building block to prepare further derivatized nucleosides such as 2',3'-dideoxycytidine. NAT10 catalyzes the formation of N4-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs.

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68498-1-IgDotBlot.pdf