NACA Polyclonal antibody, PBS Only

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• NACA Polyclonal antibody, PBS Only (32235-1-PBS)

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  WB analysis of HeLa using 32235-1-AP (same clone as 32235-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 32235-1-AP (NACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 32235-1-AP (same clone as 32235-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32235-1-PBS in a different storage buffer formulation.

NACA Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 32235-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1

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Product Information

32235-1-PBS targets NACA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag37704

  Product name: Recombinant human NACA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-215 aa of BC106041

  Sequence: MPGEATETVPATEQELPQPQAETGSGTESDSDESVPELEEQDSTQATTQQAQLAAAAEIDEEPVSKAKQSRSEKKARKAMSKLGLRQVTGVTRVTIRKSKNILFVITKPDVYKSPASDTYIVFGEAKIEDLSQQAQLAAAEKFKVQGEAVSNIQENTQTPTVQEESEEEEVDETGVEVKDIELVMSQANVSRAKAVRALKNNSNDIVNAIMELTM

• Full Name: nascent polypeptide-associated complex alpha subunit
• Calculated Molecular Weight: 215 aa, 23 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC106041
• Gene Symbol: NACA
• Gene ID (NCBI): 4666
• RRID: AB_3670227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q13765
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769).