NAGA Monoclonal antibody, PBS Only

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  WB analysis using 67684-1-Ig (same clone as 67684-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67684-1-Ig (NAGA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67684-1-PBS in a different storage buffer formulation.

NAGA Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67684-1-PBS

Clone No.2A5F3

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

Alpha galactosidase B, D22S674, GALB, NAGA

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Product Information

67684-1-PBS targets NAGA in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: NAGA fusion protein Ag7225
• Full Name: N-acetylgalactosaminidase, alpha-
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000095
• Gene Symbol: NAGA
• Gene ID (NCBI): 4668
• RRID: AB_2882877
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17050
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

NAGA belongs to the glycosyl hydrolase 27 family. It removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. It is required for the breakdown of glycolipids. Biosynthetic studies performed with cultured fibroblasts indicated that the human enzyme was synthesized as a 65 kDa glycosylated precursor which was processed to a mature 48-kDa lysosomal form; both the precursor and mature forms had high mannose type oligosaccharide chains, but only the precursor's mannose residues were phosphorylated. 90-117 kDa is a homodimer of NAGA.