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NANOS3 Polyclonal antibody, PBS Only (21679-1-PBS)

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WB analysis of mouse testis using 21679-1-AP (same clone as 21679-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 21679-1-AP (NANOS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 21679-1-AP (same clone as 21679-1-PBS)
WB result of NANOS3 antibody (21679-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-NANOS3 transfected HEK-293 cells. This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 21679-1-AP (same clone as 21679-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 21679-1-AP (NANOS3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21679-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NANOS3 Polyclonal antibody, PBS Only

Name: NANOS3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 21679-1-PBS

NANOS3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 21679-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

NOS-3, NOS3, NOS 3, NANOS1L, nanos homolog 3 (Drosophila)

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

21679-1-PBS targets NANOS3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16014 Product name: Recombinant human NANOS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-192 aa of BC101209 Sequence: MGTFDLWTDYLGLAHLVRALSGKEGPETRLSPQPEPEPMLEPVSALEPMPAPESVPVPGPKDQKRSLESSPAPERLCSFCKHNGESRAIYQSHVLKDEAGRVLCPILRDYVCPQCGATRERAHTRRFCPLTGQGYTSVYSHTTRNSAGKKLVRPDKAKTQDTGHRRGGGGGAGFRGAGKSEPSPSCSPSMST Predict reactive species
Full Name	nanos homolog 3 (Drosophila)
Calculated Molecular Weight	192 aa, 21 kDa
Observed Molecular Weight	26-30 kDa
GenBank Accession Number	BC101209
Gene Symbol	NANOS3
Gene ID (NCBI)	342977
RRID	AB_10859250
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P60323
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The NANOS gene family is required for germ cell development in diverse organisms. NANOS3 is found in migrating primordial germ cells, and the homozygous deficiency of NANOS3 in the mouse results in the complete loss of germ cells in both sexes.