NAP1L4 Polyclonal antibody

• Featured Product
• KD/KO Validated

NAP1L4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16018-1-AP

Synonyms

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

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Validation Data Gallery

• 

  WB analysis of HeLa using 16018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16018-1-AP

  WB result of NAP1L4 antibody (16018-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 16018-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16018-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16018-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16018-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16018-1-AP

  IP result of anti-NAP1L4 (IP:16018-1-AP, 4ug; Detection:16018-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human liver using 16018-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16018-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, mouse testis tissue, HEK-293 cells, A431 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Product Information

16018-1-AP targets NAP1L4 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NAP1L4 fusion protein Ag8827
• Full Name: nucleosome assembly protein 1-like 4
• Calculated Molecular Weight: 375 aa, 43 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC022090
• Gene Symbol: NAP1L4
• Gene ID (NCBI): 4676
• RRID: AB_2150711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen
human	WB	Mol Cell Proteomics; A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.; Authors - Hélène Meistermann
mouse	IF,WB	J Virol; NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.; Authors - Francisco Dominguez
human	WB,IHC	J Dermatol Sci; Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.; Authors - Satoru Mizuhashi ; KD Validated
human	WB	Nat Struct Mol Biol; Mechanism of client selection by the protein quality-control factor UBE2O; Authors - Matthew C J Yip
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi