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NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for WB, ELISA

Cat No. 27889-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 27889-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 27889-1-AP
WB result of NAP1L4 antibody (27889-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human testis using 27889-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 27889-1-AP (NAP1L4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HeLa cells, HuH-7 cells, mouse heart tissue, mouse testis tissue, rat heart tissue, U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27889-1-AP targets NAP1L4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27469 Product name: Recombinant human NAP1L4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 222-375 aa of BC022090 Sequence: TKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTITKQVPNESFFNFFNPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGEEGEEEELEGDEEGEDEDDAEINPKV Predict reactive species
Full Name	nucleosome assembly protein 1-like 4
Calculated Molecular Weight	375 aa, 43 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC022090
Gene Symbol	NAP1L4
Gene ID (NCBI)	4676
RRID	AB_2881006
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99733
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer.

Protocols

Product Specific Protocols
WB protocol for NAP1L4 antibody 27889-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The NAP1L4 Polyclonal antibody (Cat# 27889-1-AP) has 1 total citation. The cited publication appears in bioRxiv (preprint), titled "Mechanisms controlling the deposition and dynamics of histone variant H2BE," published in 2026. The research field is chromatin biology and histone variant dynamics, with the antibody applied in Western blotting for mouse samples.