NARFL Polyclonal antibody

NARFL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13652-1-AP

Synonyms

HPRN, IOP1, LET1L, NARFL, PRN, Protein related to Narf

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Validation Data Gallery

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  WB analysis using 13652-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13652-1-AP (NARFL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 13652-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 13652-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13652-1-AP targets NARFL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NARFL fusion protein Ag4217
• Full Name: nuclear prelamin A recognition factor-like
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 26-28 kDa
• GenBank Accession Number: BC030248
• Gene Symbol: NARFL
• Gene ID (NCBI): 64428
• RRID: AB_2877965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NARFL(Nuclear prelamin A recognition factor-like protein) is also named as PRN and belongs to the NARF family. This protein plays a role in cytoplasmic Fe-S protein biogenesis in mammalian cells. NARFL has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IF	Sci Rep; NARFL deficiency caused mitochondrial dysfunction in lung cancer cells by HIF-1α-DNMT1 axis; Authors - Hongzhou Liu