NARFL Polyclonal antibody

NARFL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13652-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

HPRN, IOP1, LET1L, NARFL, PRN, Protein related to Narf

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHC

WB analysis using 13652-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13652-1-AP (NARFL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 13652-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 13652-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13652-1-AP (NARFL antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13652-1-AP targets NARFL in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4217 Product name: Recombinant human NARFL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 129-476 aa of BC030248 Sequence: QLNPTDTARKLTSFFKKIGVHFVFDTAFSRHFSLLESQREFVRRFRGQADCRQALPLLASACPGWICYAEKTHGSFILPHISTARSPQQVMGSLVKDFFAQQQHLTPDKIYHVTVMPCYDKKLEASRPDFFNQEHQTRDVDCVLTTGEVFRLLEEEGVSLPDLEPAPLDSLCSGASAEEPTSHRGGGSGGYLEHVFRHAARELFGIHVAEVTYKPLRNKDFQEVTLEKEGQVLLHFAMAYGFRNIQNLVQRLKRGRCPYHYVEVMACPSGCLNGGGQLQAPDRPSRELLQHVERLYGMVRAEAPEDAPGVQELYTHWLQGTDSECAGRLLHTQYHAVEKASTGLGIRW Predict reactive species
Full Name	nuclear prelamin A recognition factor-like
Calculated Molecular Weight	476 aa, 53 kDa
Observed Molecular Weight	26-28 kDa
GenBank Accession Number	BC030248
Gene Symbol	NARFL
Gene ID (NCBI)	64428
RRID	AB_2877965
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H6Q4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NARFL(Nuclear prelamin A recognition factor-like protein) is also named as PRN and belongs to the NARF family. This protein plays a role in cytoplasmic Fe-S protein biogenesis in mammalian cells. NARFL has 3 isoforms produced by alternative splicing.